Amamus Universal PCR Master Mix (2X)

PCR ENZYMES – UNIVERSAL PCR MASTER MIX

Amamus Universal PCR Master Mix (2X) is a 2X premix composed of an optimized buffer, PCR enzyme, dNTP mixture and a density reagent. The master mix format greatly simplifies workflows and sample handling: add primers, template, water and begin PCR.

Catalog #SpecificationsSize
PCHPC0020 1.25ml (100reactions)1 tube
3 tubes

Application

  • Routine PCR
  • Colony PCR
  • Plasmid insert verification

Features

  • Easy to use, highly sensitive, accurate, and stable in performance.
  • Supports fast PCR program settings, reducing waiting time.
  • Applications: conventional PCR amplification, high-throughput PCR, and two-step RT-PCR.

Validation Data

Gel electrophoresis image showing multiple bands, indicating successful PCR amplification of DNA samples.
Diagram illustrating the 2-step Fast PCR program with temperature steps: 95°C for denaturation, 60°C for annealing, and 72°C for extension.
A table detailing PCR temperature settings, including temperature, time, and the number of cycles required for each step.

Figure. Rapid, Sensitive Reliable and Reproducibility.
The expected 498bp PCR products can be amplified with Amamus Universal PCR Master Mix from as little as 5 pg of template Lambda DNA. And three independent PCR reaction preparation were tested to show consistency of results.