About Amamus SM nuclease
Amamus SM nuclease is a non-specific nucleic acid endonuclease derived from Serratia Marcescens that degrades all forms of DNA and RNA such as double-stranded, single-stranded, linear, circular or supercoiled. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. This makes the enzyme attractive for the reduction of process related nucleic acids, such as host cell DNA and residual plasmids in biomanufacturing processes.
| Catalog # | Specifications | Size |
|---|---|---|
| PBISM0010 | 25 kU | |
| 100 kU |
Application
- Removing DNA/RNA from other biologicals
- Viscosity reduction in Lysates
- Sample preparation in electrophoresis, western blot analysis, ELISA and chromatography
Features
- The purity of Amamus SM nuclease as determined by SDS-PAGE, is ≥ 90%
- Amamus SM nuclease is a robust enzyme that remains active under a range of conditions
| Reaction condition | Optimal* | Effective** |
| Temperature | 37 °C | 0–50 °C |
| pH Value | 8.0–9.0 | 6.0–9.5 |
| Magnesium conc. | 1–2 mM | 1–10 mM |
| Monovalent cations conc. (Na⁺、K⁺) | 0–20 mM | 0–150 mM |
| Phosphate ion conc. | 0–10 mM | 0–50 mM |
Validation Data
Amamus SM nuclease exhibits comparable degradation activity for both DNA and RNA when compared to its competitors.
