cDNA SYNTHESIS – REVERSE TRANSCRIPTASE
Amamus M-MLV Reverse Transcriptase is a recombinant RNA-directed DNA polymerase that is capable for preparation of firststrand cDNA using an RNA template. The enzyme is modified to reduce RNase H activity and increase thermalstability enabling improved synthesis of full-length cDNA even for long mRNA.
| Catalog # | Concentration | Size |
|---|---|---|
| PBIMV0030 | 10,000 units | |
| PMIMV0030 | 50,000 units |
Application
- RT-PCR applications for amplification from RNA targets (e.g., RNA virus)
- Synthesis of first-strand cDNA for cloning
- cDNA library construction for RNA-Seq transcriptome studies
Features
- Amamus M-MLV Reverse Transcriptase is able to perform reverse transcription reactions throughout a wide range of temperatures from 45°C to 60°C.
Validation Data
Amamus M-MLV Reverse Transcriptase Thermal stability and performance
RT-qPCR experiments targeting nucleoprotein gene (N gene) of coronavirus were performed in triplicate over a 5-log range of input N gene RNA transcript (500 copies to 5 x 106 copies) using an initial 5 min RT step performed at 45°C – 60°C, as indicated.
Amamus M-MLV Reverse Transcriptase exhibits sensitivity, reproducibility and RT-qPCR performance
Amamus M-MLV Reverse Transcriptase was used in One-Step RT-PCR Reaction to amplify the portion of nucleoprotein gene (N gene of coronavirus). Quantified N gene RNA transcript was used as template and serial diluted to the following gradients: 5 copies to 5 x 106 copies (over 5 orders of magnitude). NTC = non-template control